Antioxidant and Antibacterial Activity of Bioactive Compound Produced By Endophytic Fungi
Keywords:Antibacterial, antioxidant, endophytic fungi, bioactive compounds
The present study aims evaluated the antibacterial and antioxidant activity of bioactive compounds synthesized by endophytic fungi obtained from Anacardium occidentale (AO), Terminalia cattappa (TC) and Sygygium jambos (SJ). The compounds produced by the isolated endophytic fungi from the medicinal plants used in this study were extracted with ethylacetate and subjected to antioxidant and antibactrerial activities using standard procedures. Screened for bioactivity using the disc diffusion method. Five fungal species were isolated from the plants and were identified to be Aspergillus terreus, Cladosporium spp, Mucor racemosus, Aspergillus oryzae, F. oxysporum. Alkaloids, saponins and steroids were detected in all the extracts while tannin and phenols were not detected. However, flavonoids was detected in SJ1 and TC2 only. All the extracts obtained from this study showed good antioxidant activity with SJ1 having the highest DPPH radical scavenging compared to the other extracts. All the extracts inhibited the growth of both E. coli and S. aureus with a range of 10-35 mm in diameter. The MBC: MIC of AO, SJ1 and SJ2 was 2.0 for both S. aureus and E. coli while that of TC2 was 10. In conclusion, the compounds produced by the isolated fungi possess both antioxidant and antibacterial activity which can be used for development of new drugs.
. Jiaojiao, S., P. Wattanachai, and S. Kasem, Isolation and identification of endophytic fungi from 10 species palm trees. Journal of Agricultural Technology, 2016. 12(2): p. 349-363.
. Siti, N.M., A.W. Sarini, et al., Isolation and Identification of Endophytic Fungi from UiTM Reserve Forest, Negeri Sembilan; Sains Malaysiana, 2018. 47(12): p. 3025–3030.
. Anchana, A., Extraction of natural dyes from fungus - an alternate for textile dyeing. Journal of Natural Sciences, 2014. 4(7): p. 1-7.
. Verma, V.C., R.N. Kharwar, and G.A. Strobel, Chemical and functional diversity of natural products from plant associated endophytic fungi. Natural Product Communications, 2009. 4(11): p. 1511-1532.
. Porras-Alfaro, A. and P. Bayman, Hidden fungi, emergent properties: Endophytes and microbiomes. Annual Review of Phytopathology, 2011. 49(1): p. 291-315.
. Martinez-Klimova, E., K. Rodríguez-Peña, and S. Sánchez, Endophytes as sources of antibiotics. Biochemical Pharmacology, 2017. 134: p. 1-17.
. Venugopalan, A. and S. Srivastava, Endophytes as in vitro production platforms of high value plant secondary metabolites. Biotechnology Advances, 2015. 33: p. 873- 887.
. Khiralla, A. et al., Endophytic fungi associated with Sudanese medicinal plants show cytotoxic and antibiotic potential. FEMS Microbiology Letters, 2016. p. 363. fnw089. 10.1093/femsle/fnw089.
. Praptiwi, I.M., A. Fathoni, D. Wulansari, and A. Agusta, Antibacterial screening of the culture of endophytic fungal extracts isolated from cinnamon stick (Cinnamomum burmannii (Nees&T.Nees) BLUME). Teknologi Indonesia, 2015. 38 (1): p. 33–41.
. Ukwatta, K.M., J.L. Lawrence, and C.D.Wijayarathne, Antimicrobial, anti-cancer, anti-filarial and anti-inflammatory activities of Cowabenzophenone A extracted from the endophytic fungus Aspergillus terreus isolated from a mangrove plant Bruguiera gymnorrhyza. Mycology, 2020. 11: p. 297–305.
. Safwan, S., et al., Bioactive compounds from an endophytic fungi Nigrospora aurantiaca. Bot Stud, 2021. 62: p. 18. https://doi.org/10.1186/s40529-021-00324-7
. Tacconelli, E., E. Carrara, et al., WHO Pathogens Priority List Working Group. Discovery, research, and development of new antibiotics: the WHO priority list of antibiotic-resistant bacteria and tuberculosis. Lancet Infect Diseases, 2018. 18(3): p. 318–327.
. Kumari, M., S. Taritla, A. Sharma, and C. Jayabaskaran, Antiproliferative and antioxidative bioactive compounds in extracts of marine-derived endophytic fungus Talaromyces purpureogenus. Front Microbiol, 2018. 9: p.1777–1789
. Looke, F.M.D., T. Gottlieb T, C.A. Jones, The global challenges of infectious diseases. Med J Aust, 2015. 202(5): p. 225–226.
. Plummer, M. et al., Global burden of cancers attributable to infections in 2012: a synthetic analysis. Lancet Global Health, 2016. 4(9): p. e609–16.
. Gitler, A.D., P. Dhillon, J. and Shorter, Neurodegenerative disease: models, mechanisms, and a new hope. Dis Model Mech, 2017. 10(5): p. 499–502.
. Omotayo, M. A., C.O. Ogundare, S.A. Salau, and S. Adenekan, In-vitro Antioxidant, Antiglycation and anti- Lipid Peroxidation activities of Sorghum bicolor Methanolic Leaf Sheath Extract. FUW Trends In Science & Technology Journal, 2021. 6 (3): p. 787 – 790.
. Pessini, G.L., B.P. Dias-Filho, C.V. Nakamura and D.A.G. Cortez, Antibacterial activity of extracts and neolignans of Piper regnellii (Miq.) C.DC. var pallescens (C.DC) Yunck. Mem. I. Oswalso Cruz, 98: p. 1115-1120.
. Barnett, H.L. and B.B. Hunter, Illustrated Genera of Imperfect Fungi. APS press. St. Paul Minneesota, USA. 1998.
. Anitha, D., T. Vijaya, D. Pragathi, et al., Isolation and characterization of endophytic fungi from endemic medicinal plants of Tirumala hills. Int J Life Sci Biotechnol Pharm Res, 2013. 2(3): p. 367-373.
. Suryanarayanan, T.S. and S. Thennarasan, Temporal variation in endophyte assemblages of Plumeria rubra leaves‟. Fungal Diversity, 2004. 15: p. 197-204.
. Sofowora, A., Medicinal Plants and Traditional Medicine in Africa'. 2014. 3rdedn. Ibadan: Spectrum
. Omotayo, M.A., S.M. Akoro, M.O. Avungbeto, and H. Uwakwe, Evaluation of Free Radical Scavenging and Antibacterial Activity of Acalypha wilkesiana and Terminalia catappa Methanolic Leaf Extracts. Microbiology Research Journal International, 2017. 19 (3): p. 1-9.
. Das, K., R.K.S. Tiwari, and D.K. Shrivastava, Techniques for evaluation of medicinal plant products as antimicrobial agent: current methods and future trends. Journal of Medicinal Plants Research, 2010. 4: p.104–111.
. Lu, Y., C. Chuan, C. Hong, et al., Isolation and Identification of Endophytic Fungi from Actinidia macrosperma and Investigation of Their Bioactivities. Evidence-Based Complementary and Alternative Medicine, 2012.
. Torres-Mendoza, D., H.E. Ortega, and L. Cubilla-Rios, Patents on endophytic fungi related to secondary metabolites and biotransformation applications. Journal of Fungi, 2020. 6(2): p. 58.
. Solomon, L., V.P. Tomii, and A.A. Dick, Importance of Fungi in the Petroleum, Agro-Allied, Agriculture and Pharmaceutical Industries. N Y Sci J , 2019. 12(5): 8-15.
. Mahan, K., R. Le, T. Wells, et al., Production of single cell protein from agro-waste using Rhodococcus opacus. Journal of Industrial Microbiology & Biotechnology, 2018. 45. 10.1007/s10295-018-2043-3.
. Olaleye, O.N., M.A. Omotayo, O. Olanlege and A.O. Longe, Shelf-Life Extension Of Tomato (Lycopersicum esculentum) And Pepper (Capsicum annum) Using Aqueous Extracts of Some Ethnomedicinal Plants. Journal of Agricultural Science and Technology, 2014. A4: p. 806-810.
. Cushnie, T.T., B. Cushnie, and A.J. Lamb, Alkaloids: An overview of their antibacterial, antibiotic-enhancing and antivirulence activities. Int. J. Antimicrob. Agents, 2014. 44: p. 377–386.
. Cowan, M., Plant products as antimicrobial agents. Clin. Microbial. Rev., 2014. 12: p. 564–582. 10.1128/CMR.12.4.564
. Ogunwenmo, K.O., O.A. Idowu, C. Innocent, et al., Cultivars of Codiaeum variegatum (L.) Blume (Euphorbiaceae) show variability in phytochemical and cytological characteristics. Journal of Biotechnology, 2007. 20: p. 2400-2405.
. El Aziz, M.M.A, A.S. Ashour, and A.S.G. Melad, A review on saponins from medicinal plants: chemistry, isolation, and determination. J Nanomed Res., 2019. 8(1): p. 282-288. DOI: 10.15406/jnmr.2019.07.00199
. Tagousop, C.N., J.D. Tamokou, et al., Antimicrobial activities of saponins from Melanthera elliptica and their synergistic effects with antibiotics against pathogenic phenotypes. Chemistry Central Journal, 2018. 12: p. 97 https://doi.org/10.1186/s13065-018-0466-6
. Saio, V. and D. Syiem, Phytochemical Analysis Of Some Traditionally Used Medicinal Plants Of North-East India. Journal of Science and Environment Today, 2015. 1: p. 6-13.
. Edeoga, H., D.E. Okwu and B. Oyedemi, Phytochemical constituents of some Nigerian Medicinal Plants. African Journal of Biotechnology, 2005. 4. 10.5897/AJB2005.000-3127.
. Al-Bayati F.A and H.F. Al-Mola, Antibacterial and antifungal activities of different parts of Tribulus terrestris L. growing in Iraq. Journal of Zhejian University Science, 2008. 9: p. 154-159.
. Ngoci S.N., C.M. Mwendia and C.G. Mwaniki, Phytochemical and cytotoxicity testing of Indigofera lupatana Baker F. Journal of Animal & Plant Sciences, 2011. 11(1): p. 1364-1373.
. Soares, M., M. Bellintani, et al., Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. European journal of pharmacology, 2003. 459: 107-12. 10.1016/S0014-2999(02)02829-7.
. Omotayo, M. A., M.O. Avungbeto, and O.O. Eleyowo, Antioxidant and Antibacterial Activity of Anacardium occidentale and Psidium guajava Methanolic Leaf Extracts. Journal of Complementary and Alternative Medical Research, 2020. 10(2): p. 29-37.
. Lambert, P.A., Cellular impermeability and uptake of biocides and antibiotics in Gram-positive bacteria and mycobacteria. J Appl Microbiology, 2002. 92S: p. 46S-54S. PMID: 12000612.
. Alves, T.M.A, A.F. Silva, et al., Biological screening of Brazilian medicinal plants. Memorias do Instituto Oswaldo Cruz., 2000. 95: p. 367–373.
. Benjamin, T.T., J.A. Adebare, et al.,s Efficiency of some disinfectants on bacterial wound pathogens. Life Science Journal, 2012. 9:2012.
How to Cite
Copyright (c) 2022 O. N. Olaleye, M. A. Omotayo, M. O. Avungbeto, O. S. Olojede, D. B. Lawal, O. B. Adeola
This work is licensed under a Creative Commons Attribution 4.0 International License.