New hybrid approach for identification of spermatozoa in human semen sample using microscope image processing techniques
DOI:
https://doi.org/10.14738/aivp.26.790Keywords:
Semen analysis, region segmentation, spermatozoa, scan line algorithmAbstract
Due to very hectic, stressful and exhaustive life style, Today, Infertility is a common problem in most of the couples and they have to undergo for intrauterine insemination (IUI) or In-vitro fertilization (IVF). Semen analysis is fundamental test in the clinical work up of the infertile male. Computerized semen test will give many more & accurate parameters that are useful to the fertility specialist. This paper focuses on finding the numbers of spermatozoa contain in the semen sample with new hybrid approach using MATLAB. Use of background subtraction is the first stage of algorithm. Region based segmentation finds the numbers of sperm in the semen sample. The comparative analysis of segmentation shows that region base segmentation detects more objects. So it is possible to find the suppressed (low intensity) sperm in given frame.References
. Kevin Coetzee, Thinus F.Kruge and Carl J.Lombard, “Predictive value of normal sperm morphology: a structured literature review”, Human Reproduction Update 1998, Vol. 4, No. 1 pp. 73–82
. WHO Laboratory manual for the examination of human semen and sperm-cervical mucus interaction. World Health Organization, 4th ed., Cambridge University Press, 1999.
. Lukasz Witkowski , “ Exam of the density of semen and analysis of sperm cell movement “,Journal of medical informatics & technology, Vol 3/2002 , ISSN 1642-6037 .
. Lukasz Witkowski , “ An automatic system for calculating basic semen parameter” , TASK QUARTERLY 8 No 2 (2004), 231–236 .
. Vahid Reza Nafisi, Mohammad Hasan Moradi, and Mohammad Hosain Nasr-Esfahani , “ Sperm Identification Using Elliptic Model and Tail Detection”, World Academy of Science, Engineering and Technology ,6 2005.
. Henry Carrillo, Jorge Villarreal, Miguel Sotaquirá ,colombia , ” Spermatozoon Segmentation Towards an Objective Analysis of Human Sperm morphology”, Proceedings of the 5th International Symposium on image and Signal Processing and Analysis (2007).
. J. M.Pascual-Gaspar, H. Olmedo, A.I. Exposito , A. Exposito3 and J.Finat1 , Spain, “A simple and effective system for Computer Assisted Semen Analysis “,Advances in Medical signal and information processing , 2008, 4th International conference, Santa Margherita Ligure, Italy, ISBN:9780 86341 934 8, 14-16 July 2008 .
. Hiromasa Oku and Masatoshi Naoko Ogawa Kogiku Shiba andManabu , japan , “How to Track Spermatozoa using High-Speed Visual Feedback “,30th Annual International IEEE EMBS Conference , Vancouver, British Columbia, Canada, August 20-24, 2008 .
. V.S.Abbiramy et. Al.,“Spermatozoa Detection, Counting and Tracking in Video Streams to Detect Asthenozoospermia “, 2010 International Conference on Signal and Image Processing.
. Chaudhari, N.M.; Pawar, B.V., "Light scattering study on semen analysis methods/techniques," Engineering (NUiCONE), 2013 Nirma University International Conference on Engineering , vol., no., pp.1,4, 28-30 Nov. 2013.
. Qiang Wu, Fatima A. Merchant, Kenneth R. Castleman, Microscope Image Processing, Academic Press, United States of America, 2008.