Isolation of Biosurfactant-Producing Bacteria from Petroleum- Polluted Mangrove Swamp Samples Using Selective Enrichment Procedure



  • C. I. Uzoigwe School of Health and Life Sciences, Teesside University Middlesbrough, Tees valley TS1 3BX, United Kingdom National Horizon Centre, Darlington, DL1 1HG, United Kingdom
  • J. Scrivens School of Health and Life Sciences, Teesside University Middlesbrough, Tees valley TS1 3BX, United Kingdom National Horizon Centre, Darlington, DL1 1HG, United Kingdom
  • A. Bello School of Health and Life Sciences, Teesside University Middlesbrough, Tees valley TS1 3BX, United Kingdom National Horizon Centre, Darlington, DL1 1HG, United Kingdom



Biosurfactant, Bacillus humi, Nutrient broth, mangrove swamp


Fifty-five morphologically distinct isolates were obtained from crude oil polluted mangrove swamp samples collected from Niger-Delta, region of Nigeria, using three microbiological media for selective enrichment. Isolation from the cultivation media was performed at 3 intervals (Days 3, 7 and 14). 35% of filamentous/irregular shaped colonies were obtained at longer incubation (Day 14) and 80% of colonies were gram positive rods. Cell-free supernatants from twenty-two isolates indicated biosurfactant activity in one or all test media (Nutrient medium (NB), NB + 2% glycerol and Mineral salt medium + 2% glucose) based on results from five screening tests; surface tension, emulsification index, oil displacement, drop collapse and haemolysis. These isolates demonstrated medium-dependent biosurfactant synthesis with 16 isolates producing biosurfactant in NB + 2% glycerol compared to other test media. Results of surface tension reduction ranged from 29.8 to 48.6 mN m-1 but results did not correlate well with emulsification index, hence combination of screening methods was essential for confirmation of biosurfactant-producing isolates. 16SrRNA sequencing identified isolates belonging to Bacillus, Serratia and Paenibacillus genera. Among these isolates, SB14-1 identified as Bacillus humi utilised three test media showing ST reduction (34.1 to 48.6 mN m-1) and emulsification of paraffin oil (49.1 to 62.6%). To date, there are no reports of biosurfactant synthesis from Bacillus humi amongst other Bacillus species. The use of selective enrichment media and combination of biosurfactant screening tests could be a successful approach towards isolation of possibly new biosurfactant-producing strains and/or hyper-producers.


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How to Cite

Uzoigwe, C. I., Scrivens, J., & Bello, A. (2022). Isolation of Biosurfactant-Producing Bacteria from Petroleum- Polluted Mangrove Swamp Samples Using Selective Enrichment Procedure: NA. European Journal of Applied Sciences, 10(4), 297–312.