Prevalence of Rotavirus among Iraqi children with diarrhea in Diyala province
DOI:
https://doi.org/10.14738/jbemi.82.10091Keywords:
Rotavirus, Diarrhea, ICA, ELISAAbstract
Background: Rotavirus is the most common cause of acute gastroenteritis in children below five years.
Objectives: The aim of this study to evaluate the prevalence of rotavirus among children with diarrhea, the efficacy of Immuno Chromatography Assay (ICA) and Enzyme-linked Immunosorbent assay (ELISA) and evaluate the relationship between the demographic agent and clinical presentation associated with rotavirus.
Material and Methods: A cross-sectional study was conducted at AL-Batool Teaching Hospital in Baqubah, from the first of April 2019 to 31 of March 2020, on (300) children with diarrhea below five years of age. The patients included (n=161, 53.6%) males and (n=139, 46.3%) females. The patient’s age, gender, living area, season, mode of feeding, source of water supply and sterilization, were collected through a questionnaire prepared for this study. The Rapid Test and ELISA are used for the detection of rotavirus in fecal specimens.
Results: The rotavirus antigen was detected in (47.33%) stool specimens by the ELISA test and (59%) by ICA from (300) children with diarrhea and high infection in the age group between 13-24 months at the rate (49.40%). The highest infection rate in spring was (56.00%) and lower in summer (33.33%). The infection males more than females (42.24% vs. 39.57%). High significant infection among children living in rural areas (55.68%) compared to those living in urban areas (35.48%). According to the clinical features, the results showed the rate of rotavirus infection in children with fever was (54.90%), vomiting (42.03%), those suffering from severe dehydration (48.85) some dehydration 78 (46.15%), however, rotavirus infection was significant with fever only.
References
2. Gastañaduy PA, Hall AJ, Parashar UD. Rotavirus. InFoodborne Infections and Intoxications 2013 Jan 1 (pp. 303-311). Academic Press.
3. P. H. Dennehy. Transmission of rotavirus and other enteric pathogens in the home. Pediatr. Infect. Dis. J., 19(10):S103–S105, 2000
4. D. M. Bass. Rotaviruses, caliciviruses, and astroviruses. Nelson Textb. Pediatr. 19th ed. Philadelphia Saunders, pages 1134–1137, 2011
5. T. L. Ferreira et al. Performance of a latex agglutination test in the diagnosis of acute gastroenteritis by rotavirus. Brazilian J. Microbiol., 37(4):587–589, 2006.
6. Cicirello HG, BK Das, A Gupta, MK Bhan, JR Gentsch, R Kumar, RI Glass. High prevalence of rotavirus infection among neonates born at hospitals in Delhi, India: predisposition of newborns for infection with unusual rotavirus. Pediatr. Infect. Dis. J. 1994
7. Husain M, PSeth, L Dar, S Broor. Classification of rotavirus into G and P types with specimens from children with acute diarrhea in New Delhi, India. J. Clin. Microbiol. 1996; 34:1592-1594
8. Fenjan MN, Jarullah BA, Abdulrahman SJ. Molecular Identification And Phylogenetic Analysis Of Rotavirus In Children Suffered From Diarrhea Under Five Years Old In Thi-Qar Provience, Of Iraq. International Journal of Pharmaceutical Research. 2020 Oct;12(4).
9. Abd-Al Fattah SA, Hamad MA, AL-Ani MQ. Prevalence and Molecular Detection of Rotavirus in Children in Ramadi City-Iraq. Medico Legal Update. 2020 Nov 18;20(4):2096-102.
10. Adnan Abdul-Ridha PN, Abdul-Razak Sh Hasan P, lecturer Ansam Salman AD. A comparison study between Enzyme-linked immunosorbant assay and rapid latex agglutination test for the diagnosis of Rotavirus in human. 2015;11(1).
11. Kheyami AM, Cunliffe NA, Hart CA. Rotavirus infection in Saudi Arabia. Annals of Saudi medicine. 2006 May;26(3):184-91.
12. Azaran A, Makvandi M, Samarbafzadeh A, Neisi N, Hoseinzadeh M, Rasti M, Teymurirad M, Teimoori A, Varnaseri M, Makvandi K. Study on Rotavirus infection and its genotyping in children below 5 years in south west iran. Iranian journal of pediatrics. 2016 Apr;26(2).
13. Chakravarti A, Chauhan MS, Sharma A, Verma V. Distribution of human rotavirus G and P genotypes in a hospital setting from Northern India. Southeast Asian J Trop Med Public Health. 2010; 41(5): 1145 -52
14. Sungkapalee T, Puntukosit P, Eunsuwan O, Theamboonlers A, Chongsrisawat V, Poovorawan Y. Incidence and clinical manifestations of rotavirus infection among children with acute diarrhea admitted at Buri Ram Hospital, Thailand. Southeast Asian J Trop Med Public Health. 2006; 37(6) : 1125 -3
15. Gladstone BP, Ramani S, Mukhopadhya I, Muliyil J, Sarkar R, Rehman AM, et al. Protective effect of natural rotavirus infection in an Indian birth cohort. N Engl J Med. 2011; 365:337-46.
16. Emerge-infect-Dis. Oct.-Dec.; 1998: 4(4); 561 -70
17. Al-Saidy AA. Role of rotavirus as the cause of acute pediatric diarrhea in Al-Diwaniyah, Iraq. Al-Qadisiyah Journal of Veterinary Medicine Sciences. 2019;18(1).
18. Hasan AR, Al-Duliami AA, AL-Zuheiry MS, Al-Azawi AK. The prevalence of rotavirus infection in Baquba-Diyala province. Diyala Journal For Pure Science. 2010;6(3).
19. Salinas B, González G, González R, Escalona M, Materán M, Schael IP. Epidemiologic and clinical characteristics of rotavirus disease during five years of surveillance in Venezuela. The Pediatric infectious disease journal. 2004 Oct 1;23(10):S161-7.
20. Abdulridha AS. Study the Effects of Age and Gender of Children on Rotavirus Infection in Baghdad, Iraq. Research Journal of Pharmaceutical Biological and Chemichal Sciences. 2016 Jul 1;7(4):700-4.
21. Suzuki H, Sakai T, Tanabe N, Okabe N. Peak rotavirus activity shifted from winter to early spring in Japan. The Pediatric infectious disease journal. 2005 Mar 1;24(3):257-60.
22. Van Thai Than WK. Prevalence of rotavirus genotypes in South Korea in 1989-2009: implications for a nationwide rotavirus vaccine program. Korean journal of pediatrics. 2013 Nov;56(11):465.
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