What Is The New Tumor Marker For Detection Of Different Kidney Tumors? Modern Study To Isolation, Purification, And Characterization Of Galactose Binding Lectin From Sera Of Patients With Kidney Cancer

  • Rasha Hasan Jasim Chemistry Department, College of Education for Girls, University of Kufa. **Chemistry Department, College of Science, University of Baghdad
  • Hathama Razooki Hasan Chemistry Department, College of Science, University of Baghdad
  • Majed Khadum Husain Biochemistry Department, College of Medicine, University of Kufa. IRAQ
Keywords: lectin, kidney, cancer, galactose, tumor, purification

Abstract

The present study was designed to investigate lectins in sera of patients with kidney tumors, in addition to non tumoral kidney disease patients. Fifty five patients of malignant kidney tumors were enrolled in addition to 23 patients of benign kidney tumors, and 18 patients of non tumoral kidney diseases used as control groups, in addition to 46 healthy individuals were also investigated. The age of patients and healthy individuals were 10-90 years. The measurement of total serum proteins revealed significant (p < 0.001) decrease in patients of malignant tumors when compared with those of benign, non tumoral diseases, and healthy individuals. The conditions of the hemagglutination assay of serum lectin activity were optimized. They were Tris buffer of 20 mM and pH 7.4, 60 mM CaCl2, 800 µg of defatted serum, 30 ˚C for serum samples, 60 minutes for serum samples, and human blood of group A+ suspension with 1.4 optical density. The measurement of the specific hemagglutination activity of lectins demonstrated significant (p < 0.001) elevation in patients of malignant tumors when compared with those of other patients and healthy individuals. Lectin activity was pointed out to be significantly (r = 0.767 at p < 0.0005) positively correlated with stage of malignancies. The cutoff value of the specific hemagglutination activity was found to equal 6 SHU for discriminatory malignant kidney tumors. Serum lectins activity were indicated to be inhibited by galactose, mannose, lactose, and N-acetyl galactosamine. Purification of lectin from sera of patients with malignant kidney tumors by affinity chromatography with the use of silver stain revealed galactose binding lectin (GalBL). The purified folds and the yield was 132 with 22.1. The polyacrylamide gel electrophoresis (PAGE) of purified lectin demonstrated one band consisted lectin activity. The approximate molecular weight of GalBL was determined and found to be 98.40. Purified lectin was characterized through the assessment of the capability to agglutinate RBCs, inhibition by EDTA, pH dependency, thermal dependent, and carbohydrate contents. GalBL was observed to be calcium independence lectins. These results suggest that the diagnosis of the specific hemagglutination activity of lectin is a promising biomarker for discrimination of malignant kidney tumor patients and the purified lectin could be introduced in the field of biomarkers.

References

Franz H. [1988]: The ricin story. Adv. Lectin Res. Vol. 1, p 10-25.

Lis H & Sharon N. [1987]: Lectins as molecules and as tools. Ann. Rev. Biochem. Vol. 55, p 35-67.

Jordan E T & Goldstein I J. [1995]: Site-directed mutagenesis studies on the lima bean lectin. Eur. Biochem J. Vol. 230, p 958-964.

Sharon N & Lis H. [2004]: History of lectins: from hemagglutinins to biological recognition molecules. Glycobiology J. Vol. 14, No. 11, p 53-62.

Hatakeyama T, Unno H, KouzumaY, Uchida T, Eto S, Hidemura H, Kato N, Yonekura M, & Kusunoki M. [2007]: C-type Lectin-like Carbohydrate Recognition of the Hemolytic Lectin CEL-III Containing Ricin-type-Trefoil Folds. Biological Chemistry J. Vol. 282, No. 52, p 37826-37835.

Kilpatrick D C. [2002]: Animal lectins: a historical introduction and overview. Biochemica et biophysica Acta J. Vol. 1572, No. 2, p 187-197.

Houzelstein D, Gonc¸alves I R, Fadden A J, Sidhu S S, Cooper D N, Drickamer K, Leffler H, & Poirier F. [2004]: Phylogenetic Analysis of the Vertebrate Galectin Family. Mol. Biol. Evol. Vol. 21, No. 7, p1177-1187.

Naird D G, Fry B G, Alewood P, Kumar P P, & Kini R M. [2007]: Antimicrobial activity of omwaprin, a new member of the waprin family of snake venom proteins. Biochem. J. Vol. 402, p 93-104.

Wong J H, Wong C C, & Ng T B. [2006]: Purification and characterization of a galactose-specific lectin with mitogenic activity from pinto beans. Biochemica et Biophysica Acta J. Vol. 1760, p 808-813.

Chandra N R, Kumar N, Jeyakani J, Singh D D, Sharan B. Gowda S B & M. N. Prathima. [2006]: Lectin db: a plant lectin database. Glycobiology J. Vol. 16, No. 10, p 938-946.

Jasim R H.[2009]:Isolation, Purification, and Partial Characterization of Human Lectints from Patients of Kidney Tumors. Baghdad University, College of Science for Women, Chemistry Department. Treatise.

Pierini C. [2007]: Lectins: Their Damaging Role in Intestinal Health, Rheumatoid Arthritis and Weight Loss. Vitamin Research News J. Vol. 21, No. 1, p 1-4.

Abd Alla M D, White G L, Rogers T B, Cary M E, Carey D W, & Ravdin J I. [2007]: Adherence-Inhibitory Intestinal Immunoglobulin A Antibody Response in Baboons Elicited by Use of a Synthetic Intranasal Lectin-Based Amebiasis Subunit Vaccine. Infection and Immunity J. Vol.

, No. 8, p 3812-3822.

Luckenbach J A, Iliev D B, Goetz F W, & Swanson P. [2008]: Identification of differentially expressed ovarian genes during primary and early secondary oocyte growth in coho salmon, Oncorhynchus kisutch. Reproductive Biology and Endocrinology. Vol. 6, No. 2, p 1-15.

Ma Y, Cheng W, Gong F, Ma A, Yu Q, Zhang J, Hu C, Chen X, & Zhang D. [2008]: Active Chinese mistletoe lectin-55 enhances colon cancer surveillance through regulating innate and adaptive immune responses Gastroenterol. World J. Vol. 14, No. 34, p 5274-5281.

Demers M, Biron-Pain K, Hebert J, Lamarre A, Magnaldo T, & St-Pierre Y. [2007]: Galectin-7 in Lymphoma: Elevated Expression in Human Lymphoid Malignancies and Decreased Lymphoma Dissemination by Antisense Strategies in Experimental Model. Cancer Res. Vol. 67, No. 6, p 2824-2829.

Yamamoto H , Nishi N, Shoji H, Itoh A, Hirashima M, & T. Nakamura. [2008]: Induction of Cell Adhesion by Galectin-8 and its Target Molecules in Jurkat T-Cells. Biochem. J. Vol. 143, No. 3, p 311-324.

van Till O J W, Modderman P W, de Boer M, Hart H L, Beld Marcel G H, & Boermeester1 M A. [2008]: Mannose-Binding Lectin Deficiency Facilitates Abdominal Candida Infections in Patients with Secondary Peritonitis. Clin. Vaccine Immunol. J. Vol. 15, No. 1, p 65-70.

Roos A, Daha M R, Pelt J, & Berger S P. [2007]: Mannose-binding lectin and the kidney. Nephrol Dial Transplant. Vol. 22, p3370-3377.

Dhuna V, Bains J S, Kamboj S S, Shanmugavel S J, & Saxena A K. [2005]: Purification and Characterization of a Lectin from Arisaema tortuosum Schott having in-vitro Anticancer Activity against Human Cancer Cell Lines. Biochemistry and Molecular Biology J. Vol. 38, No. 5, p. 526-532.

Cookson M S & Chang S S. [2007]: Malignant Tumors of the Urogenital Tract. In: Rakel: Conn’s Current Therapy. 59th Edition. W B Saunders

Miyata Y, Iwata T, Ohba K, Kanda S , Nishikido M, & Kanetake H. [2006]: Expression of Matrix Metalloproteinase-7 on Cancer Cells and Tissue Endothelial Cells in Renal Cell Carcinoma: Prognostic Implications and Clinical Significance for Invasion and Metastasis. Clin Cancer Res. Vol. 12, No. 23, p 6998-7003.

Togashi A, Katagiri T, Ashida S, Fujioka T, Maruyama O, Wakumoto Y, Sakamoto Y, Fujime M, Kawchi Y, Shuin T, & Nakamura Y. [2005]: Hypoxia-Inducible Protein 2 (HIG2), a Novel Diagnostic Marker for Renal Cell Carcinoma and Potential Target for Molecular Therapy. Cancer Res. J. Vol. 65, No. 11, p 4817-4826.

Eisenberger C F, Schoenberg M, Enger C, Hortopan S, Shah S, Chow N H, Marshall F F, & Sidransky D. [1999]: Diagnosis of Renal Cancer by Molecular Urinalysis. Journal of the National Cancer Institute, Vol. 91, No. 23, p 2028-2032.

Han W K, Alinani A, Wu C L, Michaelson D, Loda M, McGovern F J, Thadhani R, and Joseph V. Bonventre J V. [2005]: Human Kidney Injury Molecule-1 Is a Tissue and Urinary Tumor Marker of Renal Cell Carcinoma.J Am Soc Nephrol. Vol. 16, p 1126-1134.

Alamdari F I. [2007]: Renal Cell Carcinoma: Factors of importance for follow-up and survival. Sweden, Umeå, Umeå University, Urology and Andrology, Departments of Surgical and Perioperative Sciences, New Series, N1138. Thesis.

Perez-Valdivieso J R, Bes-Rastrollo M, Monedero P, de Irala J, & Lavilla F J. [2007]: Prognosis and serum creatinine levels in acute renal failure at the time of nephrology consultation: an observational cohort study. BMC Nephrology. Vol. 8, No. 14, p 1-9.

Coutinho E L, Luciana Andrade L N, Chammas, Morganti L, Nestor Schor N, & Bellini M H. [2007]: Anti-tumor effect of endostatin mediated by retroviral gene transfer in mice bearing renal cell carcinoma. The FASEB J. Vol. 21, p 3153 – 3161.

Lam J S, Belldegrun A S, & Figlin R A. [2004]: Tissue Array-Based Predictions of Pathobiology, Prognosis, and Response to Treatment for Renal Cell Carcinoma Therapy. Clinical Cancer Research. Vol. 10, p 6304s–6309s.

Bradford M. [1976]: A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein-dye binding. Anal. Biochem. J. Vol. 72, p 248-254.

Hermanson G T, Mallia A K, & Smith P K. [1992]: Immobilized Affinity Ligand Techniques. UK. Academic Press, Inc.

Dubois M, Gilles K A, Hamilton J K, Robers P A and Smith, F. [1956]: Colorimetric method for determination of sugars, and related substances. Annal. Biochem. Vol. 28, No. 3, p350-356.

Iurisci I, Tinari N, Natoli C, Angelucci D, Cianchetti E, & Iacobelli S. [2000]: Concentrations of Galectin-3 in the Sera of Normal Controls and Cancer Patients. Clinical Cancer Research. Vol. 6, p 1389-1393.

Algaba F. [2008]: Renal Adenomas: Pathological Differential Diagnosis with Malignant Tumors. Advances in Urology. Vol. 2008, p 1-4.

Indravathamma P & Seshadri H S. [1980]: Lectin from rice. Biosci. J. Vol. 2, No. 1, p 29-36.

Denis M, Palatty M, Bai N R, & Suriya S J. [2003]: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii. Eur. Biochem. J. Vol. 270, p 4348-4355.

Bulgakov A A, Eliseikina M G, Petrova IY, Nazarenko E L, Kovalchuk S N, Kozhemyako V B, & Rasskazov V A. [2007]: Molecular and biological characterization of a mannan-binding lectin from the holothurian Apostichopus japonicus. Glycobiology. Vol. 17, No. 12,

p1284-1298.

Murray P & Hall J. [2000]: Renal Replacement Therapy for Acute Renal Failure. Am. J. Vol. 162, p 777-781.

Raymond R W. [2007]: Biochemistry of Cancer. In: Cancer Biology. Section 1.p 108-120.

Cho W, Jung K, & Adamec J. [2008]: Glycoproteins Associated With Breast Cancer. Abstract from the 4th Annual USHUPO Conference, Bethesda, MD.

De Waard A, Hickman S, & Kornfeld S. [1976]: Isolation and Properties of P Galactoside Binding Lectins of Calf Heart and Lung. The Journal of Biological Chemistry. Vol. 251, No. 23, p 7581-7587.

Nambiar M P, Basu D, & Appukuttan P S. [1987]: Physicochemical properties and binding-site amino acid residues of galactoside-binding protein of human placenta. Biosci. J. Vol. 11, No. 1, p. 331-338•

Konozy E H, Bernardes E S, Rosa C, Faca V, Greene L J, & Ward R J. [2003]: Isolation, purification, and physicochemical characterization of a D-galactose-binding lectin from seeds of Erythrina speciosa. Archives of Biochemistry and Biophysics. Vol. 410, p 222-229.

Souza M A, Amâncio-Pereira F, Ribeiro C, da Silva A G, Silva E G Andrade L R, Deolina J, Lanza H, & Afonso-Cardoso S R. [2005]: Isolation and Partial Characterization of a D-Galactose-Binding Lectin from the Latex of Synadenium carinatum. Brazilian Archives of Biology and Technology. Vol. 48, No. 5, p 705-716.

Wong J H, Wong C C, & Ng T B. [2006]: Purification and characterization of a galactose-specific lectin with mitogenic activity from pinto beans. Biochemica et Biophysica Acta J. Vol. 1760, p 808-813.

Voet D, Voet J G, Pratt C W. [2006]: Fundamentals of biochemistry: life at the molecular.2nd Edition. John Wiley and Sons Inc.

Barbieri L, Falasca A, F ranceschi C, Federico Licastro F, RossiC A, & Stirpe F. [1983]: Purification and properties of two lectins from the latex of the euphorbiaceous plants Hura crepitans L. (sand-box tree) and Euphorbia characias L. (Mediterranean spurge). Biochem. J. Vol. 215, p 433-439.

Hamazak H. [1986]: Purification and Characterization of aH uman Lectin Specific for Penultimate Galactose Residues. The Journal of Biological Chemistry. Vol. 261, p 5455-5459.

Melo F R, Benevides N M, Bereira M G, Holanda M L, Meneds F N, Olivera S R, Freitas A L, & Silva L M. [2004]: Purification and partial characterization of a lectin from the red marine alga Vidalia obtusiloba C Agardh. Revista Brasil. Bot. Vol. 27, No. 2, p 263-269.

Published
2015-03-01
How to Cite
Jasim, R. H., Hasan, H. R., & Husain, M. K. (2015). What Is The New Tumor Marker For Detection Of Different Kidney Tumors? Modern Study To Isolation, Purification, And Characterization Of Galactose Binding Lectin From Sera Of Patients With Kidney Cancer. Journal of Biomedical Engineering and Medical Imaging, 2(1), 1. https://doi.org/10.14738/jbemi.21.702