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European Journal of Applied Sciences – Vol. 12, No. 3

Publication Date: June 25, 2024

DOI:10.14738/aivp.123.17113.

Naidu, K., Richards, G. A., Cruickshank, D., Wadee, B., Naidu, J., & Kajee, N. (2024). The Incidence of Fungal Infections in Patients

Treated with Tocilizumab for Severe COVID-19 Pneumonia Requiring High Care or ICU Admission: A Retrospective Cohort Study.

European Journal of Applied Sciences, Vol - 12(3). 378-388.

Services for Science and Education – United Kingdom

The Incidence of Fungal Infections in Patients Treated with

Tocilizumab for Severe COVID-19 Pneumonia Requiring High

Care or ICU Admission: A Retrospective Cohort Study

Kuven Naidu

East Rand Physicians, 36 Park Street,

Benoni, Gauteng, South Africa, 1501

Guy A Richards

University of Witwatersrand, Faculty of Health Sciences,

Johannesburg, Private Bag 3, Wits, South Africa, 2050

Deborah Cruickshank

East Rand Physicians, 36 Park Street,

Benoni, Gauteng, South Africa, 1501

Bilaal Wadee

East Rand Physicians, 36 Park Street,

Benoni, Gauteng, South Africa, 1501

Jayseelan Naidu

East Rand Physicians, 36 Park Street,

Benoni, Gauteng, South Africa, 1501

Nabeela Kajee

East Rand Physicians, 36 Park Street,

Benoni, Gauteng, South Africa, 1501

ABSTRACT

Background: Critically ill COVID-19 patients often experience immune

dysregulation, leading to cytokine release syndrome and an increased

susceptibility to nosocomial infections, including invasive fungal infections. Early

detection and treatment of fungal infections is paramount due to associated high

mortality rates. Tocilizumab, an IL-6 receptor-blocking monoclonal antibody used

in treating COVID-19 patients, has shown efficacy in managing the cytokine storm

but is linked to a heightened risk of secondary fungal infections. Aims: This study

aimed to investigate the association between Tocilizumab use and fungal

infections in COVID-19 patients admitted to an ICU in South Africa. Methods: We

conducted a retrospective cohort study at a private hospital in Johannesburg,

South Africa, from April 2020 to August 2021. Data from 373 COVID-19 patients

admitted to high care or ICU were analysed. Patients were categorized based on

positive fungal cultures. Demographics, Tocilizumab usage, and relevant factors

were collected from electronic databases. Results: Fungal infections were

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Naidu, K., Richards, G. A., Cruickshank, D., Wadee, B., Naidu, J., & Kajee, N. (2024). The Incidence of Fungal Infections in Patients Treated with

Tocilizumab for Severe COVID-19 Pneumonia Requiring High Care or ICU Admission: A Retrospective Cohort Study. European Journal of Applied

Sciences, Vol - 12(3). 378-388.

URL: http://dx.doi.org/10.14738/aivp.123.17113

identified in 15.6% of the patients. Tocilizumab use did not significantly correlate

with fungal infections (OR: 0.342 [95%CI: 0.115-1.019]; P=0.054). Factors

associated with fungal infections included extended hospital stays (OR: 1.098

[95% CI: 1.038-1.163], P=0.050 and elevated D-dimer values (OR:1.20; [95% CI:

1.035-1.390]; P=0.015). Females were less likely to develop a fungal infection

(OR:0.224; [95% CI: 0.054-0.928], P=0.039). Conclusions: Tocilizumab

administration was not significantly linked to an increased risk of fungal

infections in COVID-19 patients in the ICU. This study underscores the

multifactorial nature of fungal infection risk, emphasizing the need for

comprehensive risk assessment.

Keywords: Fungal, Tocilizumab, Covid-19, D-Dimer

INTRODUCTION

The world has recently been in the midst of the worst public health crisis since the 1918

influenza pandemic with COVID -19 officially declared to be a pandemic on March 11th 2020,

by the World Health Organisation (WHO).

It has been previously reported that 5–30% of COVID-19 patients become critically ill and

require intensive care unit (ICU) admission [1] . Severe COVID-19 is associated with immune

dysregulation, affecting both T-helper cell 2 (Th2) and Th1 responses, including the cytokine

release syndrome, which contribute to lung pathology, promote pulmonary microbial

proliferation and a subsequently secondary infection. Critically ill COVID-19 patients have

higher pro-inflammatory (IL-1, IL-2, IL-6, tumor necrosis alpha) and anti-inflammatory (IL-4,

IL-10) cytokine levels and less CD4 interferon-gamma expression, and fewer CD4 and CD8

cells [2] all of which increase the potential for nosocomial infection, including invasive fungal

infections.

Fungal infections, which could be co- or superinfections, have been reported in severely ill

COVID-19 patients admitted to the ICU, with a preponderance of aspergillus and candida

infections[3].

Awareness of the possibility of fungal superinfection in COVID-19 patients is essential to

avoid delays in diagnosis and treatment as these are associated with high mortality [5].

Coşkun et al examined the electronic health records of 627 patients hospitalized in ICU with a

diagnosis of COVID-19 [4].

Opportunistic fungal infections were detected in 32 patients (5.10%) of whom 25 (78.12%)

died and seven (21.87%) were discharged alive from the ICU. Candida parapsilosis (43.7%)

was the opportunistic fungal agent isolated most frequently from blood samples and the

mortality rate with candidemia was 80% [4].

A review of fungal infections in the United States in 2020-2021 revealed that patients

hospitalised with COVID-19 associated fungal infections had a 48.5% mortality rate compared

to 12.3% in those patients who had fungal infections without a concurrent COVID-19 infection

[5].

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European Journal of Applied Sciences (EJAS) Vol. 12, Issue 3, June-2024

Tocilizumab is a monoclonal antibody that blocks the IL-6 receptor and is registered for the

treatment of rheumatoid arthritis. It has been shown to have a significant effect on the

infection- induced cytokine storm and has been used extensively in critically ill patients

during the COVID-19 pandemic [6]. The National Institute of Health (NIH) , quoting results of

the RECOVERY [7] and REMAP-CAP trials [8], has recommended the use of this agent along

with corticosteroids in severely ill patients that are rapidly deteriorating with increasing

oxygen requirements and who have a significant inflammatory response as it offers a modest

mortality benefit.

However, an association has been reported between the use of Tocilizumab in these

circumstance and a significant increase in the risk of developing secondary fungal infections,

which increase mortality, duration of mechanical ventilation and hospital length of stay [9].

This study looks at the incidence of fungal infections in patients who had received

Tocilizumab as part of their therapy. It is our hypothesis that the incidence will be higher in

those who received Tocilizumab compared to those who did not.

METHODS

Study Design

This was a retrospective cohort study evaluating the incidence of fungal infections in patients

admitted to high care or ICU and receiving treatment with Tocilizumab for severe COVID-19

pneumonia compared to those who did not receive Tocilizumab as part of their treatment.

All patients admitted to a High care or ICU with COVID-19 pneumonia at the Life Glynnwood

Hospital (a private hospital in Johannesburg, South Africa) between 01 April 2020 and 31

August 2021, were included in the study population. The hospital numbers of all these

patients were utilised to access patient information via the electronic database (ICNET and

Impilo) in use at the hospital. This database included treatments prescribed and

microbiological culture results for each patient.

The patients were categorised into two cohorts: those with evidence of a fungal infection and

those without. A positive fungal infection was defined as a positive fungal culture on blood,

sputum or urine.

Baseline data, including demographics, the use of tocilizumab, the presence of a positive

fungal culture and length of stay were captured onto an Excel spreadsheet for review. No

personal identifiers were used.

Statistical Analysis

To assess the risk factors that could have contributed to the development of fungal infections,

unadjusted and adjusted logistic regression analyses were performed to generate odds ratios.

Variables that had a P-value below 0.2 were included in the multivariable logistic regression

models to determine significant predictors of fungal infection. A goodness-of-fit test was used

to assess the suitability of the model for analysis of the data and the likelihood ratio test was

used to compare various adjusted models to determine the best model.

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381

Naidu, K., Richards, G. A., Cruickshank, D., Wadee, B., Naidu, J., & Kajee, N. (2024). The Incidence of Fungal Infections in Patients Treated with

Tocilizumab for Severe COVID-19 Pneumonia Requiring High Care or ICU Admission: A Retrospective Cohort Study. European Journal of Applied

Sciences, Vol - 12(3). 378-388.

URL: http://dx.doi.org/10.14738/aivp.123.17113

RESULTS

Table 1: Summary statistics of patient characteristics by fungal infection post

Tocilizumab treatment

Variable No (N=315, 84.4%) Yes (N=58, 15.6%) Total (N=373) P-value

Sex Male 195 (62.1) 41 (70.7) 236 (63.4) 0.212

Female 119 (37.90 17 (29.3) 136 (36.6)

Age (years) mean (SD) 58.3 (13.0) 59.4 (10.1) 58.5 (12.6) 0.547

Diabetes

Mellitus

No 266 (84.4) 44 (75.9) 310 (83.1) 0.109

Yes 49 (15.6) 14 (24.1) 63 (16.9)

Hypertension No 208 (66.0) 29 (50.0) 237 (63.5) 0.02

Yes 107 (34.0) 29 (50.0) 136 (36.5)

CRP (mg/L) median (IQR) 107.4 (62.0-171.9) 126.4 (54.0-176.6) 110.0 (60.9-173.0) 0.723

CRP Category 0 76 (24.1) 15 (25.9) 91 (24.4)

0.458

1 82 (26.0) 10 (17.2) 92 (24.7)

2 77 (24.5) 14 (24.1) 91 (24.4)

3 80 (25.4) 19 (32.8) 99 (26.5)

CRP levels 10 mg/L or less 17 (5.5) 3 (5.7) 20 (5.5) 0.958

>10 mg/L 293 (94.5) 50 (94.3) 343 (94.5)

PCT (ng/L) median (IQR) 0.23 (0.10-0.75) 0.22 (0.09-0.62) 0.23 (0.10-0.75) 0.767

D-dimer

(mg/L)

median (IQR) 1.31 (0.76-2.68) 3.59 (1.53-6.64) 1.51 (0.79-3.63) <0.001

LOS (days) median (IQR) 16 (11-22) 22 (15-37) 16 (11-23) <0.001

Length of

stay (days)

2-7 23 (7.4) 2 (3.4) 25 (7.8)

<0.001

8-14 112 (36.1) 11 (19.0) 123 (33.4)

15-28 142 (45.8) 25 (43.1) 167 (45.4)

29 or more 33 (10.7) 20 (34.5) 53 (14.4)

Bacterial

infection

No 228 (72.4) 18 (31.0) 246 (66.0) <0.001

Yes 87 (27.6) 40 (69.0) 127 (34.0)

Deaths No 200 (63.5) 20 (34.5) 220 (59.0) <0.001

Yes 115 (36.5) 38 (65.5) 153 (41.0)

Tocilizumab

administered

No 208 (67.1) 49 (84.5) 257 (69.8) 0.008

Yes 102 (32.9) 9 (15.5) 111 (30.2)

Steroid

treatment

No steroid 2 (0.6) 0 (0.0) 2 (0.5)

0.238

methylprednisolone 206 (65.4) 35 (60.3) 241 (64.6)

Dexamethasone 43 (13.7) 4 (6.9) 47 (12.6)

Hydrocortisone 26 (8.3) 9 (15.5) 35 (9.4)

other 2 (0.6) 0.0) 2 (0.5)

missing 36 (11.4) 10 (17.2) 46 (12.3)

Descriptive Analysis

A total of 373 patients were analysed, and their epidemiological characteristics are

summarised in Table 1. Most patients were male (63.4%) (236/373). The mean age was 58

years (SD: 12.6 years) and there was no significant difference between patients who

developed fungal infection and those who did not. (P>0.05)

Diabetes and hypertension were present in 16.9% and 36.5% of patients, respectively. There

was a significant difference in the proportion of patients with hypertension among individuals

without fungal infection and those with fungal infection (P=0.020). The median CRP and PCT

were not different between patients with and without fungal infection (P>0.05).

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European Journal of Applied Sciences (EJAS) Vol. 12, Issue 3, June-2024

Although most patients had high values of D-dimer, the median D-dimer was significantly

lower among patients without fungal infection, 1.31 mg/L (IQR: 0.76-2.68) compared to those

with fungal infection, 3.59 mg/L (IQR: 1.53-6.64) (P<0.001).

The median length of stay in hospital was significantly lower among patients without fungal

infection 16 days (IQR:11-22) compared to those with fungal infection, 22 days (IQR:15-37)

(P<0.001). Close to 60% of the patients were admitted in hospital for 2 weeks or more. The

proportion of patients who also had a bacterial infection was significantly higher among those

with fungal infections (69%) compared to those without (28%) (P<0.001). The mortality was

also significantly higher among those with a fungal infection (65.5%) compared to those

without (36.5%) (P<0.001). The majority of patients received corticosteroids consisting of

methyl-prednisolone (64.6%) and dexamethasone (12.6%). The overall incidence of fungal

infections was 15.6% (N=58). The proportion of patients who developed fungal infection was

significantly lower in the group that was administered Tocilizumab (8%) compared to those

that did not receive it (19%) (P=0.01).

Age, sex, Diabetes Mellitus, CRP, and PCT values were not significantly associated with the

development of fungal infection in the unadjusted regression models (P>0.100) (Table 2).

After adjusting for potential confounders (sex, age, hypertension, CRP, PCT, D-dimer, LOS,

bacterial infection, and steroid treatment), the development of fungal infection was

significantly and negatively associated with females (OR:0.224; [95% CI: 0.054-0.928],

P=0.039), higher D-dimer values (OR:1.20; [95% CI: 1.035-1.390]; P=0.015) and longer

hospital stay (OR: 1.098 [95% CI: 1.038-1.163], P=0.050). The use of dexamethasone was

associated with an 8 times lower risk of developing a fungal infection as compared to the use

of hydrocortisone. (P<0.05).

There was a trend for Tocilizumab to reduce the odds of developing a fungal infection (OR:

0.342 [95%CI: 0.115-1.019]; P=0.054 and whereas hypertension was significantly associated

with fungal infection in the unadjusted analysis (OR: 1.916, P=0.024) it was not significantly

associated to the outcome in the final adjusted model (OR:0.910; P>0.05). Mortality was not

considered in the final model as it is not a risk factor of fungal infection, but fungal infection

was itself significantly associated with mortality.

Table 2: Logistic regression of factors associated with fungal infections.

Unadjusted logistic regression Adjusted logistic regression (full model) Parsimonious Adjusted model

Variable UOR 95% Confidence

intervals

P- value

AOR 95% Confidence

intervals

P- value

AOR 95%

Confidenc

e intervals

P- value

Sex Male 1 reference 1 reference

Female 0.679 0.369-1.250 0.214 0.224 0.054-0.928 0.039 0.296 0.098-

.0.892

0.031

Age (years) 1.007 0.984-1.031 0.545 1.040 0.990-1.092 0.119

Diabetes Mellitus No 1 reference 1 reference

Yes 1.727 0.880-3.390 0.112 2.232 0.659-7.555 0.197

Hypertension No 1 reference 1 reference

Yes 1.944 1.105-3.420 0.021 0.485 0.142-1.652 0.247

CRP 1.001 0.998-1.004 0.381 1.002 0.997-1.008 0.439

PCT 0.997 0.983-1.012 0.717 0.986 0.955-1.018 0.391

D-dimer 1.177 1.070-1.294 0.001 1.200 1.035-1.390 0.015 1.209 1.069-

1.367

0.002

Length of stay (days) 1.065 1.039-1.091 <0.001 1.098 1.038-1.163 0.001 1.072 1.029-

1.117

0.001

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Naidu, K., Richards, G. A., Cruickshank, D., Wadee, B., Naidu, J., & Kajee, N. (2024). The Incidence of Fungal Infections in Patients Treated with

Tocilizumab for Severe COVID-19 Pneumonia Requiring High Care or ICU Admission: A Retrospective Cohort Study. European Journal of Applied

Sciences, Vol - 12(3). 378-388.

URL: http://dx.doi.org/10.14738/aivp.123.17113

Bacterial

infection

No 1 reference 1 reference

Yes 5.824 3.169-10.704 <0.001 1.361 0.486-3.809 0.557

Tmab

administered

No 1 reference 1 reference

Yes 0.375 0.177-0.792 0.01 0.405 0.100-1.644 0.206 0.342 0.115-

1.019

0.054

Steroid

treatment

No steroid empty empty empty

methylprednisolo

ne

1.826 0.617-5.407 0.277 13.739 1.155-163.375 0.038 3.626 0.687-

19.129

0.129

Dexamethasone 1 reference 1 reference 1 reference

Hydrocortisone 3.721 1.040-13.310 0.043 24.844 1.541-400.626 0.024 8.006 1.158-

55.377

0.035

Table 3 gives results of a regression analysis of factors associated with fungal infection (only

fungal cultures that were positive on blood samples). It should be noted that only one patient

received Tocilizumab among the 17 who had a blood culture positive fungal infection. In the

adjusted model, only the presence of bacterial infection is significantly associated with fungal

infection (blood culture only).

Table 3: Logistic regression of factors associated with fungal infections in blood only

cultures.

Unadjusted logistic regression Adjusted logistic regression (full model) Parsimonious Adjusted

model

Variable UOR 95% Confidence

intervals

P- value

AOR 95% Confidence

intervals

P- value

AOR 95%

Confidenc

e

intervals

P- valu

e

Sex 1 1 reference 1 reference

2 0.519 0.166-1.625 0.260 Empty

Age (years) 1.005 0.967-1.045 0.799 1.016 0.920-1.121 0.757

Diabetes Mellitus No 1 reference 1 reference

Yes 1.533 0.483-4.867 0.468 2.169 0.187-25.199 0.536

Hypertension No 1 reference 1 reference

Yes 3.344 1.208-9.258 0.020 0.732 0.082-6.523 0.780

CRP 1.001 0.996-1.006 0.678 1.007 0.994-1.020 0.300

PCT 0.806 0.422-1.539 0.513 0.611 0.170-2.192 0.449

D-dimer 1.141 0.997-1.305 0.055 1.225 0.857-1.750 0.266

Length of stay (days) 1.048 1.016-1.081 0.003 1.062 0.935-1.205 0.357 1.02

9

0.996-

1.063

0.08

7

Bacterial infection No 1 reference 1 reference 1 reference

Yes 6.903 2.202-21.642 0.001 2.133 0.269-16.918 0.473 5.18

3

1.557-

17.25

0.00

7

Tocilizumab

administered

No 1 reference 1 reference

Yes 0.139 0.018-1.065 0.058 Empt

y

Steroid treatment No steroid empt

y

Empt

y

methylprednisolo

ne

1.714 0.212-13.874 0.613 0.794 0.064-9.800 0.857

Dexamethasone 1 reference 1 reference

Hydrocortisone 5.677 0.605-53.276 0.128 Empt

y

Table 4 indicates the different fungal organisms which were identified. Patients may have had

more than one fungal organism cultured during their ICU admission.

Table 4: Fungal organisms cultured.

Fungal Pathogen Number

Candida Albicans 30

Candida Glabrata 3

Candida Auris 18

Candida Parapsilosis 3

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European Journal of Applied Sciences (EJAS) Vol. 12, Issue 3, June-2024

Candida Lusitaniae 1

Candida Tropicalis 2

Canidida Species 4

Aspergillus 4

ROC Analysis of D-Dimer Values in Relation with Fungal Infection Among Patients

Admitted with COVID-19

A Receiver Operating Characteristics (ROC) analysis was conducted on 245 patients in whom

D-Dimer values were available to determine if D-dimer values could identify the presence of a

fungal infection at different cut-off points as shown in the figure 1 below. The Area Under the

Curve (AUC) was 0.71 (95% CI: 0.62 – 0.79), suggesting reasonable diagnostic accuracy with a

subjective optimal cutoff point of 1.87 with a sensitivity of 0.72, specificity of 0.63, and an AUC

of 0.68. At this cut point, 72% of patients with fungal infection and 63% without fungal

infection could be correctly identified with a Positive Likelihood Ratio (LR+) of 1.96 and a

Negative Likelihood Ratio (LR-) of 0.44. (Note: These results should only be considered within

the context of clinical guidelines and other relevant factors when making decisions about the

diagnosis and management of fungal infections).

Figure 1: ROC Analysis of D-Dimer values in relation with fungal infection among patients

admitted with COVID-19

DISCUSSION

The study describes the lack of association between tocilizumab and the development of a

fungal infection. This was contrary to our hypothesis that tocilizumab would result in an

increased incidence of fungal infections. Hospital acquired infections (HAI) in general have

previously been noted to be increased in patients with SARS-Cov-2 infection [10] [11] and in

0.00 0.25 0.50 0.75 1.00

Sensitivity

0.00 0.25 0.50 0.75 1.00

1 - Specificity

Area under ROC curve = 0.7069

For COVID-19 admitted patients in a private hospital -South Africa

ROC Analysis of D-Dimer in relation to Fungal infection post-Tocilizumab treatment

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Naidu, K., Richards, G. A., Cruickshank, D., Wadee, B., Naidu, J., & Kajee, N. (2024). The Incidence of Fungal Infections in Patients Treated with

Tocilizumab for Severe COVID-19 Pneumonia Requiring High Care or ICU Admission: A Retrospective Cohort Study. European Journal of Applied

Sciences, Vol - 12(3). 378-388.

URL: http://dx.doi.org/10.14738/aivp.123.17113

particular, were noted to be increased in COVID-19 patients that had received tocilizumab and

or steroids during the course of their treatment [12] [13] . This in particular applied to both

fungal and bacterial infections in those that had received tocilizumab [14]. This was not

surprising as it was considered that the use of immunosuppressive therapies would make

patients more susceptible to infection. However numerous studies have contradicted this

presumption. A large meta-analysis performed by the WHO Rapid Evidence Appraisal for

COVID-19 Therapies (REACT) Working Group noted that there was no significant difference

between the use of IL-6 receptor inhibitors and the development of secondary infections at 28

days (OR 0.99 CI [ 0.86-1.16])[15].

A study performed in a hospital in Milan also indicated that there was no difference in the

incidence of fungal or bacterial infection between patients who received tocilizumab and

those who did not [16] and this was mirrored by a study by Kimmig et al. which also showed

no significant association (5.6% vs 0% P=0.112). [14]. Our findings were similar as we found

no significant association between the administration of tocilizumab and the development of

a fungal infection. This is despite the fact that the incidence of fungal infections at 15.6% was

higher than in comparative studies - in an ICU in Turkey fungal infections were isolated in

5.1% of patients admitted with COVID-19 [4], an Italian hospital and Greek hospital had

incidences of 5.5% [17] and 10.7% respectively [18].

The results obtained by Atinori et al in a period in March 2020 in which 43 patients with

COVID-19 were treated with Tocilizumab and 3 (6.9%) developed candidaemia [19] were

consistent with our study in which out the 111 patients who received Tocilizumab, only 9

developed a fungal infection (8%). Overall, although our incidence of fungal infections was

higher than expected fewer of these could be attributed to the use of tocilizumab. This overall

high rate of fungal infection could be explained by other risk factors such as the prolonged use

of corticosteroids and prolonged hospitalisation. Corticosteroid use, in particular, has been

linked to the development of fungal infection and even before the results of the RECOVERY

study most patients admitted to ICU with COVID were treated with steroids. A systematic

review performed by Li et al found that corticosteroid therapy was associated with worse

outcomes for invasive and chronic pulmonary aspergillosis, invasive candidiasis and

mucormycosis [20]. It is interesting however that in our study dexamethasone appeared to be

less likely that hydrocortisone to result in a fungal infection.

Both the increased length of stay and mortality associated with fungal infections noted in our

study was in keeping with multiple previous studies in which fungi conferred a significant

negative impact on ICU outcomes. [18] [17] [4]. Furthermore, it is noted that the majority of

our patients with fungal infections had a candida as a most likely organism. Candidaemia was

noted to have been present in approximately a quarter of COVID-19 patients in a review

performed in the US during 2020 [21].

Females were noted to have a decreased risk of developing fungal infections as compared to

males. It has previously been reported that males are at a greater risk from being infected and

dying from infectious disease compared to females[22]. This may be due the positive effects of

estrogens and the negative effects of androgens on the immune system, although this is not

certain [22].

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The association between a D-Dimer and the presence of a fungal infection is interesting. There

have been previous studies which have shown an elevated D-Dimer in patients with COVID-19

infection [23, 24] and it is well documented that elevated D-dimers are also noted in patients

with venous thromboembolism, cancer and pneumonia [25]. However, there is limited data

on the significance of a positive D-dimer and associated fungal infections and this may require

further study. A correlation with positive fungal markers such as B-D-glucan and an elevated

D-Dimer would have been interesting but these values were not available. Our study has

tentatively suggested that a cut point of 1.87ug/ml for the D-dimer could be the point at

which patients should be screened for fungal infections. Whether this applies only to COVID- 19 patients is not known currently.

CONCLUSION

In conclusion, this study has shed light on the relationship between tocilizumab and the

development of fungal infections in patients with COVID-19 admitted to the ICU. Contrary to

our initial hypothesis and previous concerns about immunosuppressive therapies, we found

no significant association between tocilizumab use and the incidence of fungal infections. This

study contributes to our understanding of the complex factors influencing fungal infections in

COVID-19 patients, highlighting the need for a multifaceted approach to assess risk factors

and potential screening criteria. The use of an elevated D-Dimer as a marker for possible co- existing fungal infection is one of the potential screening factors, however further research

designed to assess the validity of its use in this regard is warranted. The study has limitations

worth considering: it was a retrospective design with a relatively small sample size, raising

concerns about selection and confounding biases. One of the major selection biases was that

the decision to administer tocilizumab may have been influenced by the patient’s condition

and other factors such as availability of the drug. The study was also not able to account for all

potential confounders that may contribute to the development of fungal infections. It is also

important to note that fungal organisms cultured from any source was included and not only

fungal organisms cultured in blood samples raising the issue of possible colonization versus

active fungal infection. Generalizability may be limited, as the findings might be specific to the

study population and the time frame. Acknowledging these limitations is crucial for a

balanced interpretation of its results.

Ethical Approval Statement: National Health Research Ethics Committee Registration: REC

251015-048

References

[1.] Peman J, Ruiz-Gaitan A, Garcia-Vidal C, Salavert M, Ramirez P, Puchades F, et al. Fungal co-infection in

COVID-19 patients: Should we be concerned? Rev Iberoam Micol. 2020;37(2):41-6.

[2.] Merad M, Martin JC. Pathological inflammation in patients with COVID-19: a key role for monocytes and

macrophages. Nat Rev Immunol. 2020;20(6):355-62.

[3.] Ezeokoli OT, Gcilitshana O, Pohl CH. Risk Factors for Fungal Co-Infections in Critically Ill COVID-19

Patients, with a Focus on Immunosuppressants. J Fungi (Basel). 2021;7(7).

[4.] Coskun AS, Durmaz SO. Fungal Infections in COVID-19 Intensive Care Patients. Pol J Microbiol.

2021;70(3):395-400.

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